Polymers and biomaterials.
Polymer and Surfactant for Biomedical Applications
In conventional tissue engineering techniques, cells are grown on tissue culture polystyrene plates; these cells are then harvested by disaggregating the extracellular matrix (ECM) through the proteolytic action of trypsin or by chelating the Ca2+ and Mg2+ ions with ethylenediaminetetraacetic acid (EDTA). However, nonspecific proteases can damage crucial cell surface proteins, resulting in decline of cell function. A new technique using a temperature-responsive polymer grafted on a cell culture substrate has been investigated as an alternative. In the past few decades, poly(N-isopropylacrylamide) (PNIAM) has been extensively studied for this application. We are interested in developing a technique for grafting the copolymer of N-isopropylarylamide and acrylamide, poly(PNIAM-co-AM), onto commercial TCP surface using ultraviolet (UV) irradiation. It is a simple grafting technique without using expensive equipment, and it also provides more choices for tissue culture researchers who can choose a method that is compatible with their cell types and their facilities.